qPCR Universal phytoplasma – 96 tests

NEW FORMAT ⁽*⁾

⁽*⁾ containing lyophilised primers and probe MIX

The qPCR Uniphy kit has been developed by Qualiplante based on Christensen et al., 2004. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.

Probes and primers for phytoplasma detection were based on alignments of 16S rDNA from a range of phytoplasma strains (one of each phytoplasma 16Sr group), bacteria, and mycoplasmas. Primers were designed to amplify DNA from a broad range of phytoplasma strains, excluding amplification of bacterial DNA.

A broad range of phytoplasma strains belonging to different subgroups of the phytoplasma 16S rDNA gene were detected using the assay. In details, the assay is specific for 16SrI-B (American aster yellows), 16SrI-C (Clover phyllody), 16SrII-A, (Sesame phyllody), 16SrIII-A (Green valley X), 16SrIII-B (Crepis biennis yellows), 16SrIII-H (Poinsettia branch-inducing), 16SrV-A (Elm yellows), 16SrV-B (Jujube witches’ broom), 16SrV-C (Alder Yellows, Grapevine yellows), 16SrV-D (Grapevine yellows), 16SrV-E (Rubus stunt), 16SrVI (Lucerne virescence), 16SrVII (Ash yellows), 16SrIX (Pichris echioides yellows), 16SrX-A (Apple proliferation), 16SrX-B (German stone fruit yellows), 16SrXI (Flower stunting), 16SrXII-A (Bois noir, Sour cherry).

No cross reactionswere observed for the following bacteria: Agrobacterium radiobacter, Arthrobacter globiformis, A. oxydans, Bacillus gibsonii, B. megaterium, Clavibacter michiganense, Paenibacillus macerans, Pseudomonas putida, Ralstonia pickettii and Rhodococcus equi.

The Universal phytoplasma specific probe is labelled with FAM^® fluorophore.