| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| nPCR APGroup 96 | nPCR APGroup 96 tests | Apple Proliferation group | Nested End-Point PCR | – | 96 tests |
The PCR AP Group kit has been developed by Qualiplante based on a method published in the Appendix 4 of PM 7/62 ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’ and ‘Ca. P. prunorum’, European and Mediterranean Plant Protection Organization Bulletin (2020) 50 (1), 69–85.
This Nested End-Point PCR method is based on a first amplification of P1/P7 primers (Deng & Hiruki, 1991; Schneider et al., 1995) that permits to confirm the presence of a phytoplasma amplifying the whole length of 16S and intergenic 16S-23S and a small part of 23S rRNA gene. FO1/rO1 primers (Lorenz et al. 1995) are used for second PCR and are specific for 16SrX group phytoplasmas.
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