NEW FORMAT (*)
(*) containing lyophilised primers and probe MIX
| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| qPCR Xfast 96 | qPCR Xfast 96 tests | Xylella fastidiosa | Real-Time PCR | FAM | 96 tests |
The qPCR Xylella fastidiosa kit has been developed by Qualiplante based on Harper and al., 2010, Erratum 2013. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The X. fastidiosa target sequence is located in the gene coding for the 16S rRNA-processing RimM protein.
The qPCR Xylella fastidiosa kit enables the detection of all the 4 main subspecies of Xylella fastidiosa:
- X. fastidiosa subsp. fastidiosa,
- X. fastidiosa subsp. multiplex,
- X. fastidiosa subsp. sandyi,
- and X. fastidiosa subsp. pauca.
without any distinction, by Real-Time PCR.
No cross reactions were observed from other bacterial species as Xanthomonas axonopodis pv. aurantifolii, X. campestris pv. citri, X. arboricola pv. fragariae, Pseudomonas syringae pv. persicae, Pantoea agglomerans, Agrobacterium tumefaciens and Spiroplasma citri.
The X. fastidiosa specific probe is labelled with FAM® fluorophore.
| Validation data | – |
| Reference | PM7/24 (4) ‘Xylella fastidiosa‘ European and Mediterranean Plant Protection Organization Bulletin (2019) 49 (2), 175–227. |
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