PCR General potyvirus – 96 tests

The PCR PotyV kit has been developed and optimized by Qualiplante according to Zheng et al., 2010 that is based on the amplification of NIb2F/NIb3R universal pair of primers designed on 2 conserved sites in the NIb (nuclear inclusion protein b) region. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.

RNA from 40 potyvirus isolates were tested. The NIb primers detected all the following potyviruses: Apium virus Y, Bean common mosaic virus, Bean yellow mosaic virus, Carrot virus Y, Ceratobium mosaic virus, Clover yellow vein virus, Cowpea aphid-borne mosaic virus, Dasheen mosaic virus, Freesia mosaic virus, Johnsongrass mosaic virus, Papaya ringspot virus, Passion fruit woodiness virus, Peanut mottle virus, Potato virus Y, Sugarcane mosaic virus, Sweet potato feathery mottle virus, Turnip mosaic virus, Watermelon mosaic virus, Zucchini yellow mosaic virus, Wheat streak mosaic virus, Banana bract mosaic virus, Onion yellow dwarf virus, Sarcochilus virus Y and Wisteria vein mosaic virus.