qPCR Flavescence doree/bois noir – 96 tests

Art. No.Product NamePathogenMethod FluorophoresSize
qPCR FDBN 96 qPCR FD BN – 96 tests Candidatus phytoplasma vitis
Candidatus phytoplasma solani		Triplex Real-Time PCR FAM, VIC, Cy5 96 tests

The qPCR FD BN kit is based on an analysis method developed by the company International Plant Analysis and Diagnostics.

The primers and probes were designed on the rpl14 FD gene and the rpl22–rps3 BN gene. The primers and probes sequences and their use in diagnostic tests are the subject of a PCT patent application by International Plant Analysis and Diagnostics. This Triplex Real-Time PCR kit offers a specific and sensitive method to detect simultaneously 16SrV phytoplasmas including FD and 16SrXII phytoplasmas including BN, as well as an endogenous control designed on the COX gene.

This qPCR FD BN kit is referenced in the Appendix 6 of the PM 7/079 (2) ‘Grapevine flavescence dorée phytoplasma’, European and Mediterranean Plant Protection Organization Bulletin (2016) 46 (1), 78–93.

qPCR FDBN 96 User Guide DOWNLOAD
Validation dataGrafdepi final report
EPPO Validation data
ReferenceAppendix 6 of the PM 7/079 (2) ‘Grapevine flavescence dorée phytoplasma’
European and Mediterranean Plant Protection Organization Bulletin (2016) 46 (1), 78–93.

Inês Medina Monteiro de Sousa Teixeira.
Estudo de expressão génica em Vitis vinifera em presença do fitoplasma Flavescência Dourada.
In: Dissertação para a obtenção do Grau de Mestre em Viticultura e Enologia, 2016.

A. Ferrandino, C. Pagliarani, O. Kedrina-Okutan, S. Icardi, M. Bove, C. Lovisolo, V. Novello and A.Schubert.
Non-anthocyanin polyphenols in healthy and Flavescence dorée infected Barbera and Nebbiolo leaves.
In: BIO Web of Conferences 13, 03003 (2019).

A. Chabirand, M. Loiseau, I. Renaudin, F. Poliakoff
Data processing of qualitative results from an interlaboratory comparison for the detection
of Flavescence dorée phytoplasma: How the use of statistics can improve the reliability of
the method validation process in plant pathology.
In: PLoS ONE 12(4): e0175247, 2017.

First report of Flavescence dorée-related phytoplasma in a productive vineyard in germany.
B. Jarausch et al.
In: Plant disease 105:3285, 2021.

M. Ripamonti, M. Pegoraro, C. Morabito, I. Gribaudo, A. Schubert, D. Bosco, C. Marzachì.
Susceptibility to flavescence dorée of different Vitis vinifera genotypes from north-western Italy.
In: Plant Pathology. 2021;70:511–520.

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