PCR Agrobacterium vitis – 96 tests

Art. No.Product NamePathogenMethod Fluorophore Size
PCR AgVit 96PCR Agrobacterium vitis – 96 testsAgrobacterium vitis & Agrobacterium tumefaciensTriplex End-Point PCR–96 tests

The PCR Agrobacterium vitis kit has been developed by Qualiplante and permits to discriminate Agrobacterium vitis from Agrobacterium tumefaciens. This Triplex End-Point method uses a combination of 3 specific primers sets that permit to amplify: the chromosomal polygalacturonase gene pehA used to identify A. vitis strains and to distinguish them from A. tumefaciens (466 bp DNA fragment), a region of virF gene coding for A. vitis nopaline and octopine pTi (382 bp) and a region of virD gene coding for A. vitis vitopine.

PCR AgVit 96 User Guide DOWNLOAD
Validation data–
ReferenceM. DUBCEAC, E. HAUSTOV, V. BONDARCIUC (2024).
Implementarea metodei PCR pentru identificare tulpinilor patogene allozhirobium vitis
ce provoaca cancerul bacterian al vitei-de-vie.
In: Agricultural Sciences, n°1, pp 47-54.

Laura Soledad Bree (2015).
Metodología para la detección de Agrobacterium vitis a partir de material vegetal de Vitis vinífera.
In: TESIS DE GRADO.

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