| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| qPCR PPV 96 | qPCR PPV 96 tests | Plum pox virus | One-Step Real-Time RT-PCR | FAM | 96 tests |
The qPCR Plum pox virus kit has been developed by Qualiplante based on Olmos et al., 2005. A verification was performed by Qualiplante (data not published) and the performance characteristics of the kit are the same as the original publication.
The assay was developed for the detection of the main types of PPV: PPV-M and PPV-D, without any distinction. To design appropriate primers and probes, the nucleotide sequence flanked by the universal primers P1 and P2 (Wetzel et al., 1991) was selected. The specific amplification of the target sequence is a 76 bp DNA fragment. This sensitive method was applied successfully to plant material and to individual PPV vector.
| Validation data | Appendix 8 EPPO PM7/32 ‘Plum pox virus’. European and Mediterranean Plant Protection Organization Bulletin (2023);53:518–539. |
| Reference | Appendix 8 EPPO PM7/32 ‘Plum pox virus’. European and Mediterranean Plant Protection Organization Bulletin (2023);53:518–539. |
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