qPCR Ralstonia solanacearum – 96 tests

NEW FORMAT ⁽*⁾

⁽*⁾ containing lyophilised primers and probe MIX

The qPCR Rsol kit has been developed by Qualiplante based on Weller and al., 2000. A verification was performed by Qualiplante and the performance characteristics of the kit are the same as the original publication.

The broad-host-range R. solanacearum probe (RS-P) is partially homologous to 16S rRNA gene primer OLI1 (24), with primers (RS-I and RS-II) flanking this region. This kit permits the detection of all strains of R. solanacearum and R. pseudosolanacearum and the specific detection and identification of R. solanacearum Phylotype II sequevar 1 (race 3) only. The broad-range probe (RS-P) detected all biovars of R. Solanacearum.

In pure culture, detection of Ralstonia solanacearum to ≥ 10^-2 cells ml-1 was achieved; sensitivity decreased when the assay was performed with inoculated potato tissue extracts, prepared by currently recommended extraction procedures.

No cross reactions were observed with Ralstonia pickettii, Ralstonia sp., Burkholderia andropogonis, Burkholderia caryophylli, Burkholderia cepacia, Burkholderia glumae, Burkholderia plantarii, Bacilus polymyxa, Pseudomonas marginalis subsp. marginalis, Pseudomonas chlorophis, Enterobacteriaceae, Ratinella aquatillis, Ochrobactrum anthropic, but cross reactions were observed with Banana blood disease bacterium, Ralstonia syzygii strains, Pseudomonas sp. (Taxom B).