| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| PCR PepMV 96 | PCR PepMV 96 tests | Pepino mosaic virus | One-Step End-Point RT-PCR | – | 96 tests |
The PCR Pepino mosaic virus kit has been developed by Qualiplante based on Ling et al. (2008). The primer pair was designed on the TGB2-3 genomic region.
The primers were designed on the capsid protein gene. They are based on CMV-Q RNA 3 and amplify an 870 bp fragment for all CMV isolates, which can be cut by the restriction enzyme MspI to obtain patterns distinguishing S-I from S-II isolates.The PCR PepMV kit enables the detection of the 4 strain groups: European (EU), US1, US2 and Ch2.
Validation data of the method are available from a study realized in 2012 during Progetto Aron-Arnadia – Armonizzazione Protocollo diagnostico per Pepino mosaic virus (PepMV) su pomodoro, Progetto Strateco. The performance characteristics obtained are:
▪ Diagnostic sensitivity: 91%.
▪ Diagnostic specificity: 100%.
▪ Accuracy: 93%.
▪ Analytical sensitivity: 10-5 for seeds, 10-8 for fruit pulp, 10-6 for leaves.
▪ Analytical specificity: 100%.
▪ Repeatability: 100%.
▪ Reproducibility: 98%.
| Validation data | – |
| Reference | – |
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