| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| nPCR Xfast 96 | nPCR Xfast 96 tests | Xylella fastidiosa | Nested End-Point PCR | – | 96 tests |
The PCR Xfast kit is based on the amplification of a part of two conserved genes (16S rRNA gene and partial sequence of gyrB gene) and has been optimized by Qualiplante.
The PCR kit was tested on different strains of X. fastidiosa isolated from different plant hosts as grapevine, citrus, coffee, plum, hibiscus, almond, elm, ragweed, and non-targeted organisms, confirming 100% of specificity.
No cross-reactions were observed for: Xanthomonas albilineans, Xanthomonas axonopodis pv. citri, Xanthomonas bromi, Xanthomonas campestris pv. campestris, Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. cassavae, Xanthomonas campestris pv. manihotis, Xanthomonas campestris pv. citri, Xanthomonas campestris pv. malvacearum, Xanthomonas campestris pv. citrumelo, Xanthomonas campestris pv. pruni, Xanthomonas campestris pv. phaseoli, Xanthomonas cordiae, Xanthomonas curcubitae, Xanthomonas hyacinthi, Xanthomonas melonis, Xanthomonas translucens pv. translucens, Xanthomonas vasicola pv. holcicola, Curtobacterium flaccunfaciens, Enterobacter cloacae, Methylbacterium extorquens, Methylbacterium mesophilicum, Methylbacterium radiotolerans, Methylbacterium zatmanii, Pantoea agglomerans, Pseudomonas aeruginosa, Pseudomonas aureofaciens, Pseudomonas choloraphis, Pseudomonas fluorescens, Pseudomonas mendocina, Pseudomonas putida, Pseudomonas syringae pv. syringae, Agrobacterium tumefaciens, Candidatus liberibater africanus, Erwinia carotovora, Escherichia coli.
| Validation data | – |
| Reference | – |
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