The kit composition depends on the molecular method used and contains at least: a ready-to-use master mix tube, positive and negative control tubes, instructions for use and a certificate of quality of the batch.
Taq-Man® qPCR
Taq-Man® qPCR method uses DNA as starting material. The amplified DNA is measured in real time after each amplification cycle using fluorescent TaqMan® probes.
One-Step Taq-Man® RT-qPCR
One-Step Taq-Man® RT-qPCR method uses RNA as starting material. In a one-step reaction, the RNA is reverse transcribed (RT) into cDNA which is amplified during the same reaction and after each amplification, the fluorescence is measured by using fluorescent Taq-Man® probes.
SYBR®-Green qPCR
SYBR®-Green qPCR method uses DNA as starting material. The amplified DNA is measured in real time after each amplification cycle using the fluorescent SYBR®-Green dye.
SYBR®-Green RT-qPCR
SYBR®-Green RT-qPCR method uses RNA as starting material. In a one-step reaction, the RNA is first reverse transcribed (RT) into cDNA, then the cDNA is amplified during the same reaction and the fluorescence is measured by using the fluorescent SYBR®-Green dye.
End-Point PCR
End-Point PCR method uses DNA as starting material. After PCR amplification, the presence or absence of the corresponding DNA product is determined by gel electrophoresis.
One-Step End-Point RT-PCR
One-Step End-Point RT-PCR uses RNA as starting material. In a one-step reaction, the RNA is first reverse transcribed (RT) into cDNA. After PCR amplification, the presence or absence of the corresponding cDNA product is determined by gel electrophoresis.
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