| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| nPCR Botr 96 | nPCR Botr – 96 tests | Botryosphaeria sp. | Nested End-Point PCR | – | 96 tests |
The PCR Botryosphaeria sp. kit has been developed by Qualiplante and permits to detect all 17 species of Botryosphaeriaceae which have been reported as potential wood pathogens of grapevine.
This Nested End-Point PCR method is based on a first amplification of primer sets designed within the regions flanked by the universal primers ITS5 and ITS4, and on a second amplification of primer sets aimed at specifically amplifying the DNA of all the species in Botryosphaeriaceae, including Neofusicoccum parvum and Neofusicoccum ribis, which have been associated with wood diseases of grapevine.
| Validation data | – |
| Reference | L. MARTÍN, F. Javier CASTAÑO, G. DURANTE, C. CHATILLON, J. LUQUE. A new molecular diagnostic tool for the detection of Botryosphaeriaceae species. Cost Action FA 1303 Workshop – « Grapevine trunk diseases: current state and future prospects » Cognac France: 23-24 June 2015. Poster Abstract L. Martín, F. Fontaine, F.J. Castaño, A. Songy, R. Roda, J. Vallet, Raú., Ferrer-Gallego. Specific profile of Tempranillo grapevines related to Esca-leaf symptoms and climate conditions In: Plant Physiology et Biochemistry (2018). |
REQUEST OF QUOTATION