| Art. No. | Product Name | Pathogen | Method | Fluorophore | Size |
|---|---|---|---|---|---|
| qPCR CTV 96 | qPCR CTV 96 tests | Citrus tristeza virus | One-Step Real-Time RT-PCR | FAM | 96 tests |
The qPCR Citrus tristeza virus kit was developed in collaboration with the company International Plant Analysis and Diagnostics.
Primers and probe were designed according to the sequence of the most conserved region of the CTV genome. The assay was evaluated on most than 400 CTV isolates from different areas (Spain, Italy, Chile, Egypt…). No cross-reactions were observed for other citrus virus and viroids: Citrus psorosis virus, Citrus vein enation virus, Citrus variegation virus, Citrus exocortis viroid, Hop stunt viroid, Citrus bent leaf viroid, Citrus viroid III and Citrus viroid IV.
Qualiplante/IpadLab qPCR kit is referred in the Appendix 6 of the PM 7/31 (2) ‘Citrus tristeza virus”, European Plant Protection Organization, Bulletin (2023); 53:42-61.The kit was evaluated in the framework of the EU funded VALITEST project and all the performance of the kit is detailed in the method.
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